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Home > News > Wide application of chromatographic techniques leads to headspace bottle cleaning
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Wide application of chromatographic techniques leads to headspace bottle cleaning

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At present, with the increasing attention to food quality and safety, chromatographic analysis technology is increasingly used in food quality and safety testing, especially in the field of agricultural product testing, chromatographic analysis technology has been widely used. In China, a large number of agricultural products (other chemical products, organic acids, etc.) are detected every year by liquid chromatography and gas chromatography. Due to the large number of samples, a large number of headspace bottles need to be cleaned during the test, which not only wastes time, but also reduces work efficiency, and sometimes there is a case where the test results are deviated due to the cleanliness of the cleaned Sample Vials.

The headspace chromatography sample bottle is made of glass and is rarely made of plastic. Single-use injection bottles are costly, wasteful, and pollute the environment. Most laboratories use the sample bottles for cleaning and reuse. At present, the main methods used in the laboratory to clean the sample bottles are to add washing powder, detergent, organic solvent and acid and alkali lotion, and then brush with a custom small tube. This conventional brushing method has many disadvantages, the detergent and water are used in a large amount, the washing time is long, and it is easy to leave a dead angle. If it is a plastic injection bottle, it is easy to leave a brush mark on the inner bottle wall, which takes up a lot of human resources. For glassware with serious lipid and protein residue contamination, alkaline lysate is used for cleaning, and good results are obtained.

Headspace bottle cleaning step

1. Open the aluminum cover in the fume hood, pour the material that can be poured out of the bottle into the waste bottle, and then let the bottle evaporate for a period of time in the fume hood;
2, put the bottle into the basin, add detergent and tap water, soak, it should not be too long, the residual material in the bottle can be cleaned;
3. Brush the bottle with a test tube brush and clean the tap water;
4. Drain the water in the bottle as much as possible and soak it for 0.5-1h with analytically pure methanol. (The soaked methanol can be reused. If the methanol is too dirty, it should be replaced.)

5. Pour the soaked methanol back into the original glass bottle and continue to use it next time. The soaked bottle is washed with tap water, then rinsed with ultrapure water for 2-3 times, rinsed and dried.


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